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Tuesday, 13 February 2018

Extracting DNA from a banana

Aim: I want to investigate how to extract DNA from a Banana

Materials
Measuring cylinder
Cold 95 percent ehanol
Universal indicator solution
Boling tube with rubber bung
Hooked wire on handle
Petri dish

Method:
1 first you have to place 50g of banana and 100 ml distilled water in the food blender and switch on 'High'  for 20 seconds.

2 Pour the blended banana mixture through a piece of clean muslin into a beaker.

3 Measure out  15 ml of the blended banana mix pour into a boiling tube. Add 6 drops of dishwashing detergent, put a thick rubber bung into the tube and shake for at least five minutes. (Mixture should become thick and gooey)

4 Carefully add 15ml of cold alcohol to the tube of banana mixture.The alcohol forms a layer on top of the blended banana.

5 To collect the DNA, dip a hooked wire into the mixture where the layers meet, then turn the wire to 'twist DNA and place on to a clean petri dish. Watch carefully as you lift the DNA from the mixture.

6 Add some universial indicator solution to your DNA.

Results
1 what does it look like?

2 what is pH of DNA         

Is DNA acidic, basic or neutral?

3 what is the full name of DNA?

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